Factor affecting enzyme kinetics :
Factor affecting enzyme kinetics : 1. Temperature : Rate of the reaction increases with increase in temperature for enzymatic reaction up to the certain limit because substrate colloidal with active site of enzyme when molecule moves rapidly above that temperature speed of enzymatic reaction drop down sharply because enzymes are made up of proteins and the proteins get the denature outside the certain range of the temperature, The optimal minimum temperature is 40 degree Celsius. The enzyme become inactive temperature over 70 degree The temperature dependence of enzymatic reaction is usually asymmetric. Increase in temperature increases the thermal movement of molecules Initially used to rate acceleration At certain temperature enzymes become unstable And activity loss as a result of denaturation 2. PH : ...
